Seed storage proteins are one of the most important food resources that play important role on bread making quality, in this respect distinguishing the genetic diversity was crucial in breeding aims. In order to study genetic diversity in 20 wheat commercial varieties with different growth type, we analysed the banding patterns of gliadin proteins by SDS-PAGE technique. Our results showed 11 different bands that were polymorphic in 20 studding varieties. Cluster analysis classified these genotypes into 3 groups. These differentiated groups had different growth types; also cluster analysis based on Jaccard similarity coefficient showed that some lines had higher similarities than the others. Principle coordinate analysis showed that 80% of total variance was determined by the first 3 components and cophenetic correlation coefficient equal to 0.75 was resulted and this confirmed the significant relation between gliadins and growth type. Existence of relation between growth type and gliadin proteins could help breeders in variety discrimination and selection perspectives.